Hotspot

ndhF-rpl32

SSC · 117509-118704 · 1196 bp

Back to Syndiclis

Pi 0.00125

MarkerSeek score 59.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1196
variable_sites: 3
pi: 0.00125
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAGCAAAAGGT	GCCTGACATCCCTTTCCTTT	50.0	50.0	58.0	57.8	1328-1330 bp	1.0	78.5
ndhF-rpl32_p2	CGGAACAAACAGAAGTCCCA	GCCTGACATCCCTTTCCTTT	50.0	50.0	58.0	57.8	1366-1368 bp	1.0	78.3
ndhF-rpl32_p3	CTGGAAGTGGAGCAAAAGGT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1271-1273 bp	1.0	77.8
ndhF-rpl32_p4	CGGAACAAACAGAAGTCCCA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1309-1311 bp	1.0	77.6
ndhF-rpl32_p5	TCGGAACAAACAGAAGTCCC	GCCTGACATCCCTTTCCTTT	50.0	50.0	57.8	57.8	1367-1369 bp	1.0	76.6