Hotspot

ycf4-cemA

LSC · 65072-65986 · 915 bp

Back to Syndiclis

Pi 0.00347

MarkerSeek score 62.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 6
pi: 0.00347
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCGGAATTGG	AGATCCACCAGGGCAAAAAG	45.0	50.0	57.8	58.1	1032-1040 bp	1.0	79.4
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1033-1041 bp	1.0	79.3
ycf4-cemA_p3	ACAAAAAGCTGCGGAATTGG	GATCCACCAGGGCAAAAAGA	45.0	50.0	57.8	58.1	1031-1039 bp	1.0	79.3
ycf4-cemA_p4	ACAAAAAGCTGCGGAATTGG	ATGCAAGATATGGGAGGGGA	45.0	50.0	57.8	57.9	1005-1013 bp	1.0	78.9
ycf4-cemA_p5	TTTCTTGCGCGTTCCAATTG	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.2	58.1	1008-1016 bp	1.0	78.7