Hotspot

ycf60

Genome · 66642-67259 · 618 bp

Back to Synarthrophyton

Pi 0.136

MarkerSeek score 80.20

Primer available yes

Label ycf60

Feature Metadata

label_name: ycf60
feature_type: gene
region: Genome
length_bp: 618
variable_sites: 83
pi: 0.136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf60_p1	GCAGCTCCTAAACGAATTGC	TGCCACTGATTGAGCTCTTC	50.0	50.0	57.8	57.9	1589 bp	0.5	57.7
ycf60_p2	GCAGCTCCTAAACGAATTGC	GCAGCGCAACCAATATCTTC	50.0	50.0	57.8	57.9	1906 bp	0.5	57.7
ycf60_p3	GCAGCTCCTAAACGAATTGC	TAACAGCAGCGCAACCAATA	50.0	45.0	57.8	58.2	1911 bp	0.5	57.1
ycf60_p4	GCAGCTCCTAAACGAATTGC	AGCAGCGCAACCAATATCTT	50.0	45.0	57.8	58.2	1907 bp	0.5	56.6
ycf60_p5	GCAGCTCCTAAACGAATTGC	TCAGCATTTTTGCATCCAGC	50.0	45.0	57.8	57.6	1675 bp	0.5	55.6