Hotspot

ycf56

Genome · 74882-75571 · 690 bp

Back to Synarthrophyton

Pi 0.241

MarkerSeek score 78.80

Primer available yes

Label ycf56

Feature Metadata

label_name: ycf56
feature_type: gene
region: Genome
length_bp: 690
variable_sites: 166
pi: 0.241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf56_p1	ATAGTTTCTCCTGGCTGTGC	CAGCTGGTTCATGGGCTTTA	50.0	50.0	57.6	58.2	1315 bp	0.5	55.4
ycf56_p2	ATAGTTTCTCCTGGCTGTGC	GTTTTGGCAGCTGGTTCATG	50.0	50.0	57.6	58.2	1322 bp	0.5	55.0
ycf56_p3	CCTGGCTGTGCATGTACTTT	CAGCTGGTTCATGGGCTTTA	50.0	50.0	58.5	58.2	1306 bp	0.5	55.0
ycf56_p4	CCTGGCTGTGCATGTACTTT	GTTTTGGCAGCTGGTTCATG	50.0	50.0	58.5	58.2	1313 bp	0.5	54.6
ycf56_p5	ACCAACATTCATGCCTGGAT	CAGCTGGTTCATGGGCTTTA	45.0	50.0	57.5	58.2	1690 bp	0.5	54.4