Hotspot

ycf29

Genome · 108268-108918 · 651 bp

Back to Synarthrophyton

Pi 0.236

MarkerSeek score 79.60

Primer available yes

Label ycf29

Feature Metadata

label_name: ycf29
feature_type: gene
region: Genome
length_bp: 651
variable_sites: 153
pi: 0.236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf29_p1	ATTATTCGCGGGTTCGAGTC	GTTTTTGTGATTGCGGTTGC	50.0	45.0	58.2	57.4	966 bp	0.5	53.6
ycf29_p2	ATTATTCGCGGGTTCGAGTC	TTGTAACATTCACGCGTTGG	50.0	45.0	58.2	57.3	1226 bp	0.5	53.4
ycf29_p3	CAAGGGGTTAAGGCAATGGA	GTTTTTGTGATTGCGGTTGC	50.0	45.0	57.8	57.4	996 bp	0.5	53.0
ycf29_p4	CAAGGGGTTAAGGCAATGGA	TTGTAACATTCACGCGTTGG	50.0	45.0	57.8	57.3	1256 bp	0.5	52.8
ycf29_p5	GCTAACCTCAACCTCCTCCT	GTTTTTGTGATTGCGGTTGC	55.0	45.0	58.4	57.4	1318 bp	0.5	51.4