Hotspot

ycf21

Genome · 54326-54850 · 525 bp

Back to Synarthrophyton

Pi 0.25

MarkerSeek score 69.50

Primer available yes

Label ycf21

Feature Metadata

label_name: ycf21
feature_type: gene
region: Genome
length_bp: 525
variable_sites: 7
pi: 0.25
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf21_p1	ACCCAATGACATGAATGGTA	CGTCAGATAAGAATTGAATTTTCT	40.0	29.2	54.3	53.7	740 bp	0.5	20.0
ycf21_p2	TACCCAATGACATGAATGGT	CGTCAGATAAGAATTGAATTTTCT	40.0	29.2	54.3	53.7	741 bp	0.5	20.0
ycf21_p3	ACCCAATGACATGAATGGTA	CGTCAGATAAGAATTGAATTTTCTTCT	40.0	29.6	54.3	56.7	740 bp	0.5	20.0
ycf21_p4	TACCCAATGACATGAATGGT	CGTCAGATAAGAATTGAATTTTCTTCT	40.0	29.6	54.3	56.7	741 bp	0.5	20.0
ycf21_p5	ACCCAATGACATGAATGGTA	CGTCAGATAAGAATTGAATTTTCTTC	40.0	30.8	54.3	55.5	740 bp	0.5	20.0