Hotspot

upp

Genome · 98652-99251 · 600 bp

Back to Synarthrophyton

Pi 0.278

MarkerSeek score 79.70

Primer available yes

Label upp

Feature Metadata

label_name: upp
feature_type: gene
region: Genome
length_bp: 600
variable_sites: 165
pi: 0.278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
upp_p1	ACAGCCATGGCAAGAAAGAA	GCTCGTCCATCTCTTCGTTT	45.0	50.0	58.0	58.0	1798 bp	0.5	60.0
upp_p2	ATGAGATCACCTAGTGGCGA	GCTCGTCCATCTCTTCGTTT	50.0	50.0	57.9	58.0	1926 bp	0.5	59.4
upp_p3	TCCATTGGTTACGCAAGCTT	GCTCGTCCATCTCTTCGTTT	45.0	50.0	58.1	58.0	1271 bp	0.5	59.2
upp_p4	CTGCTGGCTTTGAGAAAGGA	GCTCGTCCATCTCTTCGTTT	50.0	50.0	58.1	58.0	1595 bp	0.5	59.2
upp_p5	ATCCATTGGTTACGCAAGCT	GCTCGTCCATCTCTTCGTTT	45.0	50.0	57.9	58.0	1272 bp	0.5	59.0