Hotspot

rps5

Genome · 136505-137056 · 552 bp

Back to Synarthrophyton

Pi 0.164

MarkerSeek score 80.40

Primer available yes

Label rps5

Feature Metadata

label_name: rps5
feature_type: gene
region: Genome
length_bp: 552
variable_sites: 88
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps5_p1	TGAAAATCCACCGCCAGAAA	GTTGGAGAAGACATTGGCCA	45.0	50.0	58.0	58.1	891 bp	0.5	59.2
rps5_p2	TTGAAAATCCACCGCCAGAA	GTTGGAGAAGACATTGGCCA	45.0	50.0	58.0	58.1	892 bp	0.5	59.2
rps5_p3	TGAAAATCCACCGCCAGAAA	AGTTGGAGAAGACATTGGCC	45.0	50.0	58.0	57.8	892 bp	0.5	58.3
rps5_p4	TTGAAAATCCACCGCCAGAA	AGTTGGAGAAGACATTGGCC	45.0	50.0	58.0	57.8	893 bp	0.5	58.3
rps5_p5	TGAAAATCCACCGCCAGAAA	GGCAAAGGTATACGCTACGA	45.0	50.0	58.0	57.5	1140 bp	0.5	55.9