Hotspot

orf456

Genome · 156791-158155 · 1365 bp

Back to Synarthrophyton

Pi 0.319

MarkerSeek score 79.70

Primer available yes

Label orf456

Feature Metadata

label_name: orf456
feature_type: gene
region: Genome
length_bp: 1365
variable_sites: 432
pi: 0.319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf456_p1	GGCCCAGCTCATATTGGTAC	TCTTGAGAACCCCTAGCCAA	55.0	50.0	58.1	58.0	2210 bp	0.5	58.9
orf456_p2	GGCCCAGCTCATATTGGTAC	TTCTTGAGAACCCCTAGCCA	55.0	50.0	58.1	58.0	2211 bp	0.5	58.9
orf456_p3	GGCCCAGCTCATATTGGTAC	AACCCTCCAACCTCACGATA	55.0	50.0	58.1	58.1	2681 bp	0.5	58.8
orf456_p4	GGCCCAGCTCATATTGGTAC	ACCCTCCAACCTCACGATAA	55.0	50.0	58.1	58.1	2680 bp	0.5	58.8
orf456_p5	ATGCACCACTAGGGGATGAT	TCTTGAGAACCCCTAGCCAA	50.0	50.0	58.2	58.0	2146 bp	0.5	58.3