Hotspot

orf160

Genome · 100379-100876 · 498 bp

Back to Synarthrophyton

Pi 0.305

MarkerSeek score 76.60

Primer available yes

Label orf160

Feature Metadata

label_name: orf160
feature_type: gene
region: Genome
length_bp: 498
variable_sites: 152
pi: 0.305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf160_p1	AAACGAAGAGATGGACGAGC	TTGTTCCAGGTGGAGGATCT	50.0	50.0	58.0	58.0	1437 bp	0.5	59.6
orf160_p2	AAACGAAGAGATGGACGAGC	GTTCCAGGTGGAGGATCTCT	50.0	55.0	58.0	58.1	1435 bp	0.5	59.0
orf160_p3	AAACGAAGAGATGGACGAGC	ATGTTGGGTACAGCAAGACG	50.0	50.0	58.0	58.2	1720 bp	0.5	58.4
orf160_p4	CGAAGAGATGGACGAGCAAT	TTGTTCCAGGTGGAGGATCT	50.0	50.0	57.8	58.0	1434 bp	0.5	57.9
orf160_p5	AAACGAAGAGATGGACGAGC	TGATCCCGCGAAAGTAACTC	50.0	50.0	58.0	57.7	1181 bp	0.5	57.7