Hotspot

hisS

Genome · 102781-104040 · 1260 bp

Back to Synarthrophyton

Pi 0.222

MarkerSeek score 79.40

Primer available yes

Label hisS

Feature Metadata

label_name: hisS
feature_type: gene
region: Genome
length_bp: 1260
variable_sites: 278
pi: 0.222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
hisS_p1	TTTTAATGCGGCCTGTCGTA	ATCCATAAACTGGGGTGGGA	45.0	50.0	57.9	58.0	1938 bp	0.5	59.2
hisS_p2	TTAATGCGGCCTGTCGTATC	ATCCATAAACTGGGGTGGGA	50.0	50.0	58.1	58.0	1936 bp	0.5	58.8
hisS_p3	TTTTAATGCGGCCTGTCGTA	TAAACTGGGGTGGGAGGATT	45.0	50.0	57.9	57.9	1933 bp	0.5	58.7
hisS_p4	TTTTAATGCGGCCTGTCGTA	CTTTGGGCTGACCGTTGATA	45.0	50.0	57.9	57.9	2285 bp	0.5	58.4
hisS_p5	TTTTAATGCGGCCTGTCGTA	CTTACCACTTGGCTACACCC	45.0	55.0	57.9	57.9	1879 bp	0.5	58.4