Hotspot

chlB

Genome · 156154-159705 · 3552 bp

Back to Synarthrophyton

Pi 0.237

MarkerSeek score 75.60

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 3552
variable_sites: 836
pi: 0.237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	TGCAGTTACAATGTTGGAGACA	TCTTGAGAACCCCTAGCCAA	40.9	50.0	58.2	58.0	777 bp	0.5	42.2
chlB_p2	TGCAGTTACAATGTTGGAGACA	TTCTTGAGAACCCCTAGCCA	40.9	50.0	58.2	58.0	778 bp	0.5	42.2
chlB_p3	TGCAGTTACAATGTTGGAGACA	TCGGCTTCAAAACCATACGT	40.9	45.0	58.2	57.8	650 bp	0.5	41.1
chlB_p4	TGCAGTTACAATGTTGGAGACA	CGGCTTCAAAACCATACGTG	40.9	50.0	58.2	57.8	649 bp	0.5	40.6
chlB_p5	TGCAGTTACAATGTTGGAGACA	AACCCCTAGCCAATACATGC	40.9	50.0	58.2	57.6	770 bp	0.5	39.6