Hotspot

ccdA

Genome · 70154-70885 · 732 bp

Back to Synarthrophyton

Pi 0.262

MarkerSeek score 79.70

Primer available yes

Label ccdA

Feature Metadata

label_name: ccdA
feature_type: gene
region: Genome
length_bp: 732
variable_sites: 190
pi: 0.262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccdA_p1	AAATTTCCACTGACGTGCAC	AGGATAGGCGAGGGTACTTC	45.0	55.0	57.2	58.0	1669-1941 bp	1.0	82.2
ccdA_p2	AAATTTCCACTGACGTGCAC	GGTTCAAATCCTTCCTGGGG	45.0	55.0	57.2	58.1	1150-1153 bp	1.0	81.0
ccdA_p3	AAATTTCCACTGACGTGCAC	TACCAATTTCACCACGTCCG	45.0	50.0	57.2	58.2	1486-1758 bp	1.0	80.6
ccdA_p4	AAATTTCCACTGACGTGCAC	ACGCCAAACCATCCAATGTA	45.0	45.0	57.2	57.8	1788-2062 bp	1.0	80.5
ccdA_p5	AAATTTCCACTGACGTGCAC	TACGCCAAACCATCCAATGT	45.0	45.0	57.2	57.8	1789-2063 bp	1.0	80.5