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acsF-petN

Genome · 160811-161262 · 452 bp

Back to Synarthrophyton

Pi 0.303

MarkerSeek score 80.70

Primer available yes

Label acsF-petN

Feature Metadata

label_name: acsF-petN
feature_type: igs
region: Genome
length_bp: 452
variable_sites: 131
pi: 0.303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acsF-petN_p1	TGCAGTGCATGATCTTTCCA	AGACTGATTGCCCATTCCAC	45.0	50.0	57.8	57.9	1139 bp	0.5	57.3
acsF-petN_p2	TGCAGTGCATGATCTTTCCA	AGAGCCCCATCCTAAACTGA	45.0	50.0	57.8	57.7	746 bp	0.5	56.2
acsF-petN_p3	AAGCCCGAAAATTCTGCAGT	AGACTGATTGCCCATTCCAC	45.0	50.0	58.4	57.9	1153 bp	0.5	55.9
acsF-petN_p4	TGCAGTGCATGATCTTTCCA	AGCCCCATCCTAAACTGACA	45.0	50.0	57.8	58.3	744 bp	0.5	55.7
acsF-petN_p5	TGCAGTGCATGATCTTTCCA	GAGCCCCATCCTAAACTGAC	45.0	55.0	57.8	57.7	745 bp	0.5	55.7