Hotspot

accB-ycf45

Genome · 152898-153305 · 408 bp

Back to Synarthrophyton

Pi 0.279

MarkerSeek score 79.60

Primer available yes

Label accB-ycf45

Feature Metadata

label_name: accB-ycf45
feature_type: igs
region: Genome
length_bp: 408
variable_sites: 107
pi: 0.279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB-ycf45_p1	TCGCATTAGAGCTTGTCCAC	CTTGCATACGCCTTGCTCTA	50.0	50.0	58.0	58.1	1396-1402 bp	1.0	88.8
accB-ycf45_p2	AATGACCAGGTTTTGCCCAT	CTTGCATACGCCTTGCTCTA	45.0	50.0	58.0	58.1	1690 bp	0.5	59.3
accB-ycf45_p3	ATGACCAGGTTTTGCCCATT	CTTGCATACGCCTTGCTCTA	45.0	50.0	58.0	58.1	1689 bp	0.5	59.3
accB-ycf45_p4	TCGCATTAGAGCTTGTCCAC	TGGAAAACGAGCTTCTGGTC	50.0	50.0	58.0	58.1	996 bp	0.5	59.0
accB-ycf45_p5	ATGACCAGGTTTTGCCCATT	TGGAAAACGAGCTTCTGGTC	45.0	50.0	58.0	58.1	1283 bp	0.5	58.8