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ycf37-chlN

Genome · 86827-87410 · 584 bp

Back to Synarthrophyton

Pi 0.31

MarkerSeek score 82.10

Primer available yes

Label ycf37-chlN

Feature Metadata

label_name: ycf37-chlN
feature_type: igs
region: Genome
length_bp: 584
variable_sites: 174
pi: 0.31
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37-chlN_p1	TCGGGATATAGCGCAGTTTG	TGATGCGCAGGAGAAAAACT	50.0	45.0	57.9	58.1	1746 bp	0.5	58.0
ycf37-chlN_p2	GTCGGGATATAGCGCAGTTT	TGATGCGCAGGAGAAAAACT	50.0	45.0	57.8	58.1	1747 bp	0.5	58.0
ycf37-chlN_p3	TCGGGATATAGCGCAGTTTG	TACAACATTGATGCGCAGGA	50.0	45.0	57.9	57.9	1754 bp	0.5	58.0
ycf37-chlN_p4	GTCGGGATATAGCGCAGTTT	TACAACATTGATGCGCAGGA	50.0	45.0	57.8	57.9	1755 bp	0.5	57.9
ycf37-chlN_p5	TCGGGATATAGCGCAGTTTG	ACATTGATGCGCAGGAGAAA	50.0	45.0	57.9	58.2	1750 bp	0.5	57.4