Hotspot

ycf1

SSC · 126325-131946 · 5622 bp

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Pi 0.00889

MarkerSeek score 49.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5622
variable_sites: 117
pi: 0.00889
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTCTGTTCAAATTCTTGGT	AGACTAGATTATGTAATGATGAGACT	28.6	30.8	52.0	55.1	213 bp	1.0	43.8
ycf1_p2	TTTTCTGTTCAAATTCTTGGT	AGACTAGATTATGTAATGATGAGAC	28.6	32.0	52.0	53.8	213 bp	1.0	43.8
ycf1_p3	TTTTCTGTTCAAATTCTTGGT	GACTAGATTATGTAATGATGAGACT	28.6	32.0	52.0	53.8	212 bp	1.0	43.8
ycf1_p4	ATTTTCTGTTCAAATTCTTGGT	AGACTAGATTATGTAATGATGAGACT	27.3	30.8	52.7	55.1	214 bp	1.0	43.7
ycf1_p5	TTTTCTGTTCAAATTCTTGGT	AAGACTAGATTATGTAATGATGAGACT	28.6	29.6	52.0	55.8	214 bp	1.0	43.7