Hotspot

rps16

LSC · 5137-6266 · 1130 bp

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Pi 0.00708

MarkerSeek score 46.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1130
variable_sites: 17
pi: 0.00708
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TACGAATCATCACTCCCCCG	TGTACCGACTGAACCGATGA	55.0	50.0	59.0	58.5	1240-1246 bp	1.0	70.0
rps16_p2	CATCACTCCCCCGCTTTTT	TGTACCGACTGAACCGATGA	52.6	50.0	58.0	58.5	1233-1239 bp	1.0	69.4
rps16_p3	TACGAATCATCACTCCCCCG	ATGTACCGACTGAACCGATG	55.0	50.0	59.0	57.4	1241-1247 bp	1.0	69.1
rps16_p4	CATCACTCCCCCGCTTTTT	ATGTACCGACTGAACCGATG	52.6	50.0	58.0	57.4	1234-1240 bp	1.0	68.5
rps16_p5	TACGAATCATCACTCCCCCG	GTACCGACTGAACCGATGAT	55.0	50.0	59.0	57.1	1239-1245 bp	1.0	66.8