Hotspot

rpl16

LSC · 83974-85426 · 1453 bp

Back to Symplocos

Pi 0.00874

MarkerSeek score 49.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1453
variable_sites: 27
pi: 0.00874
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCAAGAACTATTTCCTTTGGT	TCAAAACAAAAACGAGCTCT	39.1	35.0	57.7	54.0	1556-1566 bp	1.0	41.7
rpl16_p2	TCCCAAGAACTATTTCCTTTGGT	TCAAAACAAAAACGAGCTCTT	39.1	33.3	57.7	54.8	1556-1566 bp	1.0	41.7
rpl16_p3	TCCCAAGAACTATTTCCTTTGGT	ATCAAAACAAAAACGAGCTCT	39.1	33.3	57.7	54.6	1557-1567 bp	1.0	41.7
rpl16_p4	CCCAAGAACTATTTCCTTTGGT	TCAAAACAAAAACGAGCTCT	40.9	35.0	56.2	54.0	1555-1565 bp	1.0	41.7
rpl16_p5	TCCCAAGAACTATTTCCTTTGGT	ATCAAAACAAAAACGAGCTCTT	39.1	31.8	57.7	55.4	1557-1567 bp	1.0	41.7