Hotspot

ndhF

SSC · 113074-115326 · 2253 bp

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Pi 0.00729

MarkerSeek score 42.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 38
pi: 0.00729
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	ACTTTCGGCATCTTCATCAA	50.0	40.0	58.0	55.4	2698-2784 bp	1.0	62.0
ndhF_p2	GCAGAATACCGTCACCCATT	ACTTTCGGCATCTTCATCAA	50.0	40.0	58.0	55.4	2801-2887 bp	1.0	61.9
ndhF_p3	AAGAGGGATCCACCGAAGAA	ACTTTCGGCATCTTCATCAA	50.0	40.0	58.0	55.4	2690-2776 bp	1.0	61.9
ndhF_p4	AGCCTCCCTGAAGCTTCTTA	ACTTTCGGCATCTTCATCAA	50.0	40.0	58.0	55.4	2599-2618 bp	0.857143	54.2
ndhF_p5	TAGCCTCCCTGAAGCTTCTT	ACTTTCGGCATCTTCATCAA	50.0	40.0	58.0	55.4	2600-2619 bp	0.857143	54.2