Hotspot

trnN-GUU-trnR-ACG

IRa · 132330-132919 · 590 bp

Back to Symplocos

Pi 0.00991

MarkerSeek score 61.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 590
variable_sites: 14
pi: 0.00991
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	726-746 bp	1.0	81.4
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	763-783 bp	1.0	80.7
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	677-697 bp	1.0	80.4
trnN-GUU-trnR-ACG_p4	GGTTGAGAACGGGAATGGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	765-785 bp	1.0	79.7
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	714-734 bp	1.0	79.7