Hotspot

ndhF-rpl32

SSC · 115327-116101 · 775 bp

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Pi 0.0127

MarkerSeek score 57.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 775
variable_sites: 20
pi: 0.0127
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGTATGATCCACGCATATTGA	GGATGCCCAATATCCTTTCCT	40.9	47.6	57.1	57.5	881-895 bp	1.0	47.9
ndhF-rpl32_p2	AAAAGGTATGATCCACGCAT	GGATGCCCAATATCCTTTCCT	40.0	47.6	55.1	57.5	884-898 bp	1.0	47.8
ndhF-rpl32_p3	AAGGTATGATCCACGCATATTGA	GGATGCCCAATATCCTTTCCT	39.1	47.6	57.8	57.5	882-896 bp	1.0	45.2
ndhF-rpl32_p4	GGTATGATCCACGCATATTGA	GGATGCCCAATATCCTTTCCT	42.9	47.6	55.6	57.5	880-894 bp	1.0	44.0
ndhF-rpl32_p5	AGGTATGATCCACGCATATTG	GGATGCCCAATATCCTTTCCT	42.9	47.6	55.6	57.5	881-895 bp	1.0	43.9