Hotspot

trnR-ACG-trnN-GUU

IRb · 111049-111638 · 590 bp

Back to Symplocos

Pi 0.00991

MarkerSeek score 61.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 590
variable_sites: 14
pi: 0.00991
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	726-746 bp	1.0	81.4
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	763-783 bp	1.0	80.7
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	677-697 bp	1.0	80.4
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GGTTGAGAACGGGAATGGAA	55.0	50.0	57.8	57.8	765-785 bp	1.0	79.7
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	714-734 bp	1.0	79.7