Hotspot

petA-psbJ

LSC · 65524-66499 · 976 bp

Back to Symplocos

Pi 0.0167

MarkerSeek score 53.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 31
pi: 0.0167
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTCGAGAAGGTTCA	CTGGATTGGGTTCATCTCTGT	40.9	47.6	57.6	57.4	1017-1043 bp	1.0	52.2
petA-psbJ_p2	TCGAGAAGGTTCAATTGTCTGA	CTGGATTGGGTTCATCTCTGT	40.9	47.6	57.4	57.4	1008-1034 bp	1.0	50.7
petA-psbJ_p3	AGAAACAGTTCGAGAAGGTTCA	TGGATTGGGTTCATCTCTGT	40.9	45.0	57.6	56.1	1016-1042 bp	1.0	50.3
petA-psbJ_p4	AGAAACAGTTCGAGAAGGTTCA	TCTGGATTGGGTTCATCTCTG	40.9	47.6	57.6	57.1	1018-1044 bp	1.0	50.0
petA-psbJ_p5	TCGAGAAGGTTCAATTGTCTGA	TGGATTGGGTTCATCTCTGT	40.9	45.0	57.4	56.1	1007-1033 bp	1.0	48.8