Hotspot

ycf3-trnS-GGA

LSC · 46354-47174 · 821 bp

Back to Symplocos

Pi 0.00974

MarkerSeek score 57.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 20
pi: 0.00974
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	910-924 bp	1.0	72.8
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	931-945 bp	1.0	72.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	881-895 bp	1.0	72.0
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	911-925 bp	1.0	70.4
ycf3-trnS-GGA_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	908-922 bp	1.0	69.7