Hotspot

trnT-GGU-psbD

LSC · 33224-34744 · 1521 bp

Back to Symplocos

Pi 0.00739

MarkerSeek score 55.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1521
variable_sites: 23
pi: 0.00739
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGTAACCAGTCATCCATAA	55.0	45.0	58.1	54.6	1648-1652 bp	1.0	53.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGTAACCAGTCATCCATAA	50.0	45.0	58.1	54.6	1651-1655 bp	1.0	53.4
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCGTAACCAGTCATCCATAA	50.0	45.0	58.3	54.6	1625-1629 bp	1.0	51.8
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	CCGTAACCAGTCATCCATAA	50.0	45.0	57.7	54.6	1663-1667 bp	1.0	51.7
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	CCGTAACCAGTCATCCATAA	50.0	45.0	58.4	54.6	1647-1651 bp	1.0	50.6