Hotspot

petN-psbM

LSC · 29605-30755 · 1151 bp

Back to Symplocos

Pi 0.0082

MarkerSeek score 64.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 21
pi: 0.0082
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CGTAGTATGGGGAAGAAGCG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	1257-1258 bp	1.0	69.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1312-1313 bp	1.0	68.9
petN-psbM_p3	TAGTATGGGGAAGAAGCGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.5	57.6	1255-1256 bp	1.0	66.4
petN-psbM_p4	GTAGTATGGGGAAGAAGCGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.5	57.6	1256-1257 bp	1.0	65.9
petN-psbM_p5	CGTAGTATGGGGAAGAAGCG	TGCTACTGCACTGTTCATTC	55.0	45.0	57.9	56.1	1257-1258 bp	1.0	64.9