Hotspot

trnG-GCC-trnR-UCU

LSC · 10470-10729 · 260 bp

Back to Symplocos

Pi 0.00916

MarkerSeek score 55.60

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 5
pi: 0.00916
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TCTGGAAAAATTTGCGTCCA	50.0	40.0	58.0	56.2	388-411 bp	1.0	68.8
trnG-GCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TCTGGAAAAATTTGCGTCCA	55.0	40.0	58.1	56.2	391-414 bp	1.0	68.5
trnG-GCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	340-363 bp	1.0	67.7
trnG-GCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	343-366 bp	1.0	67.4
trnG-GCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	TCTGGAAAAATTTGCGTCCAA	50.0	38.1	58.0	56.9	388-411 bp	1.0	66.9