Hotspot

trnS-GCU-trnG-GCC

LSC · 9040-9707 · 668 bp

Back to Symplocos

Pi 0.00984

MarkerSeek score 66.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 14
pi: 0.00984
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCTTTAACT	45.0	40.0	58.0	54.5	777-781 bp	1.0	53.8
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	739-743 bp	1.0	53.8
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	742-746 bp	1.0	52.7
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	741-745 bp	1.0	52.7
trnS-GCU-trnG-GCC_p5	ATTAGCAATCCGCCGCTTTA	AAACCGAAAGACCCTTTAACT	45.0	38.1	58.0	55.3	778-782 bp	1.0	52.6