Hotspot

trnK-UUU-rps16

LSC · 4323-5136 · 814 bp

Back to Symplocos

Pi 0.00881
MarkerSeek score 55.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
814
variable_sites
17
pi
0.00881
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAGGCGGAGGTTTTTAAGGA 50.0 45.0 58.2 57.0 703-909 bp 1.0 76.5
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AGGCGGAGGTTTTTAAGGAA 50.0 45.0 58.2 57.0 702-908 bp 1.0 76.5
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAGGCGGAGGTTTTTAAGGA 50.0 45.0 58.2 57.0 702-908 bp 1.0 76.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGCGGAGGTTTTTAAGGAA 50.0 45.0 58.2 57.0 701-907 bp 1.0 76.5
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC AGGCGGAGGTTTTTAAGGAA 50.0 45.0 58.3 57.0 717-923 bp 1.0 75.7