Hotspot

psbA

LSC · 321-1382 · 1062 bp

Back to Symplocarpus

Pi 0

MarkerSeek score 35.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGCTTGGTATTGCTCCTTCA	TAGCTAATACGTGCGCTTGG	50.0	50.0	57.9	58.2	1165 bp	1.0	77.8
psbA_p2	GGCTTGGTATTGCTCCTTCA	CGCTTGGGAGTCCTTGAAAA	50.0	50.0	57.9	58.4	1152 bp	1.0	75.8
psbA_p3	AGAGGCTTGGTATTGCTCCT	TAGCTAATACGTGCGCTTGG	50.0	50.0	58.4	58.2	1168 bp	1.0	75.5
psbA_p4	AGGCTTGGTATTGCTCCTTC	TAGCTAATACGTGCGCTTGG	50.0	50.0	57.6	58.2	1166 bp	1.0	75.4
psbA_p5	GAGGCTTGGTATTGCTCCTT	TAGCTAATACGTGCGCTTGG	50.0	50.0	57.6	58.2	1167 bp	1.0	75.4