Hotspot

ndhK

LSC · 50352-51098 · 747 bp

Back to Symplocarpus

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label ndhK

Feature Metadata

label_name
ndhK
feature_type
gene
region
LSC
length_bp
747
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK_p1 TGTTATTCTGCGCAAAAGCA GGTTCAGTTTATGCATGGCG 40.0 50.0 56.9 57.8 808 bp 1.0 69.9
ndhK_p2 TGTTATTCTGCGCAAAAGCA TGGGCAATGAGTTTCGATGT 40.0 45.0 56.9 57.8 883 bp 1.0 69.9
ndhK_p3 TGTTATTCTGCGCAAAAGCA AGTTTCGATGTATTGGGCGT 40.0 45.0 56.9 57.6 874 bp 1.0 68.3
ndhK_p4 TGTTATTCTGCGCAAAAGCA GATGTATTGGGCGTATCCGT 40.0 50.0 56.9 57.5 868 bp 1.0 67.9
ndhK_p5 TGTTATTCTGCGCAAAAGCA GTTCAGTTTATGCATGGCGC 40.0 50.0 56.9 58.5 807 bp 1.0 67.9