Hotspot

ndhF

SSC · 112698-114914 · 2217 bp

Back to Symplocarpus

Pi 0.00135

MarkerSeek score 58.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 3
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAACGACATATGTATGTGAAATT	AGAGTTGGTGAATCGGAAACA	26.1	42.9	52.8	57.5	2387-2389 bp	1.0	40.1
ndhF_p2	AAACGACATATGTATGTGAAATT	GAGTTGGTGAATCGGAAACA	26.1	45.0	52.8	56.0	2386-2388 bp	1.0	40.1
ndhF_p3	AAACGACATATGTATGTGAAATT	AAGAGTTGGTGAATCGGAAACA	26.1	40.9	52.8	58.2	2388-2390 bp	1.0	40.1
ndhF_p4	AAACGACATATGTATGTGAAATT	AGAGTTGGTGAATCGGAAACAA	26.1	40.9	52.8	58.2	2387-2389 bp	1.0	40.1
ndhF_p5	ACGACATATGTATGTGAAATTAA	AGAGTTGGTGAATCGGAAACA	26.1	42.9	52.1	57.5	2385-2387 bp	1.0	40.1