Hotspot

GFC13_pgt029-ndhF

IRb · 111497-112697 · 1201 bp

Back to Symplocarpus

Pi 0.000837

MarkerSeek score 59.70

Primer available yes

Label GFC13_pgt029-ndhF

Feature Metadata

label_name: GFC13_pgt029-ndhF
feature_type: igs
region: IRb
length_bp: 1201
variable_sites: 1
pi: 0.000837
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
GFC13_pgt029-ndhF_p1	AGGATTCGAACCTACGACCA	ATCTAGGGGGTGGACGAATC	50.0	55.0	58.2	58.3	1353-1354 bp	1.0	76.9
GFC13_pgt029-ndhF_p2	AATCAGTTAACAGCCGACCG	ATCTAGGGGGTGGACGAATC	50.0	55.0	58.3	58.3	1334-1335 bp	1.0	75.9
GFC13_pgt029-ndhF_p3	ACCACTGAGCTACTGAGGAA	ATCTAGGGGGTGGACGAATC	50.0	55.0	57.7	58.3	1310-1311 bp	1.0	75.7
GFC13_pgt029-ndhF_p4	CCCCAAGTAGGATTCGAACC	ATCTAGGGGGTGGACGAATC	55.0	55.0	57.7	58.3	1361-1362 bp	1.0	75.5
GFC13_pgt029-ndhF_p5	CGAACCTACGACCAATCAGT	ATCTAGGGGGTGGACGAATC	50.0	55.0	57.4	58.3	1347-1348 bp	1.0	73.0