Hotspot

rpoA-rps11

LSC · 81129-81196 · 68 bp

Back to Symplocarpus

Pi 0.0147
MarkerSeek score 61.00
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
LSC
length_bp
68
variable_sites
1
pi
0.0147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 GAGTGGATGCTGCTACTTCC AAGAGACGCAGCATTACGAG 55.0 50.0 58.1 58.1 214 bp 1.0 79.3
rpoA-rps11_p2 GTGGATGCTGCTACTTCCTC AAGAGACGCAGCATTACGAG 55.0 50.0 58.1 58.1 212 bp 1.0 79.3
rpoA-rps11_p3 GAGTGGATGCTGCTACTTCC GAAGAGACGCAGCATTACGA 55.0 50.0 58.1 58.1 215 bp 1.0 79.2
rpoA-rps11_p4 GTGGATGCTGCTACTTCCTC GAAGAGACGCAGCATTACGA 55.0 50.0 58.1 58.1 213 bp 1.0 79.2
rpoA-rps11_p5 TGCTACTTCCTCTCGAACCA AAGAGACGCAGCATTACGAG 50.0 50.0 58.1 58.1 204 bp 1.0 79.1