Hotspot

petA-psbJ

LSC · 64957-66001 · 1045 bp

Back to Symplocarpus

Pi 0.00481

MarkerSeek score 62.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1045
variable_sites: 5
pi: 0.00481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGCGTCCAAGGTCTTTT	CTGTGATCGGTTCAATCGGT	45.0	50.0	57.8	58.0	1224-1226 bp	1.0	78.7
petA-psbJ_p2	GCGTCCAAGGTCTTTTGTTC	CTGTGATCGGTTCAATCGGT	50.0	50.0	57.6	58.0	1220-1222 bp	1.0	77.4
petA-psbJ_p3	GTTACGCGTCCAAGGTCTTT	CTGTGATCGGTTCAATCGGT	50.0	50.0	58.5	58.0	1225-1227 bp	1.0	76.7
petA-psbJ_p4	TACGCGTCCAAGGTCTTTTG	CTGTGATCGGTTCAATCGGT	50.0	50.0	58.5	58.0	1223-1225 bp	1.0	76.7
petA-psbJ_p5	TTACGCGTCCAAGGTCTTTT	TCCTGTGATCGGTTCAATCG	45.0	50.0	57.8	57.7	1226-1228 bp	1.0	76.4