Hotspot

atpB-rbcL

LSC · 55703-56497 · 795 bp

Back to Symplocarpus

Pi 0.00252

MarkerSeek score 60.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 2
pi: 0.00252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACAGAACGAGAAGTTGCAGG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	874-876 bp	1.0	71.2
atpB-rbcL_p2	CAGAACGAGAAGTTGCAGGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	873-875 bp	1.0	71.0
atpB-rbcL_p3	ACAGAACGAGAAGTTGCAGG	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	873-875 bp	1.0	70.9
atpB-rbcL_p4	CAGAACGAGAAGTTGCAGGA	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.8	58.1	872-874 bp	1.0	70.7
atpB-rbcL_p5	AAAAGCGACATCCAGTACCG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.3	58.0	947-949 bp	1.0	70.0