Hotspot

psaA-ycf3

LSC · 43050-43722 · 673 bp

Back to Symplocarpus

Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	824 bp	1.0	76.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	CCGATCAAGCTGCTGAGTAT	50.0	50.0	57.9	57.5	853 bp	1.0	75.2
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TCCGATCAAGCTGCTGAGTA	50.0	50.0	57.9	58.5	854 bp	1.0	75.1
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GAAATTGCAGAGGCTTGGTC	50.0	50.0	57.9	57.4	872 bp	1.0	74.2
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGGAAACAAGCTATAGCGCT	50.0	45.0	57.9	57.3	833 bp	1.0	73.8