Hotspot

trnC-GCA-petN

LSC · 28892-29979 · 1088 bp

Back to Symplocarpus

Pi 0.0046

MarkerSeek score 61.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 5
pi: 0.0046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTGATTGTTTTGGCGGCATG	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.2	58.2	1196-1197 bp	1.0	77.5
trnC-GCA-petN_p2	TTGATTGTTTTGGCGGCATG	AGAGTCCACTTCTTCCCCAT	45.0	50.0	58.2	57.6	1257-1258 bp	1.0	75.9
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	1161-1162 bp	1.0	75.0
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.5	57.6	1222-1223 bp	1.0	73.4
trnC-GCA-petN_p5	ATTGATTGTTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	40.0	50.0	57.2	58.2	1197-1198 bp	1.0	73.1