Hotspot

clpP

LSC · 66151-68096 · 1946 bp

Back to Symphytum

Pi 0.00822

MarkerSeek score 53.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1946
variable_sites: 16
pi: 0.00822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGTTTAGATTGATCCTAACCGG	ACGTTTCCACATCAAAGTGA	45.5	40.0	56.3	55.5	2118-2134 bp	1.0	40.6
clpP_p2	GGTTTAGATTGATCCTAACCGG	ACGTTTCCACATCAAAGTGAA	45.5	38.1	56.3	56.3	2118-2134 bp	1.0	40.6
clpP_p3	GGTTTAGATTGATCCTAACCGG	ACGTTTCCACATCAAAGTGAAA	45.5	36.4	56.3	57.0	2118-2134 bp	1.0	40.6
clpP_p4	TCCTAACCGGATGATTTGAT	ACGTTTCCACATCAAAGTGA	40.0	40.0	53.8	55.5	2106-2122 bp	1.0	40.6
clpP_p5	TCCTAACCGGATGATTTGATT	ACGTTTCCACATCAAAGTGA	38.1	40.0	54.7	55.5	2106-2122 bp	1.0	40.6