Hotspot

ycf2-trnI-CAU

IRa · 145871-146009 · 139 bp

Back to Symphytum

Pi 0.0458

MarkerSeek score 83.10

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 139
variable_sites: 6
pi: 0.0458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TCCAGGATTCGAGTGGATGT	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	199 bp	1.0	84.5
ycf2-trnI-CAU_p2	AGGATTCGAGTGGATGTCCT	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.8	58.0	196 bp	1.0	84.3
ycf2-trnI-CAU_p3	CAGGATTCGAGTGGATGTCC	GTTGGGCGCTTTAACCATTC	55.0	50.0	57.8	58.0	197 bp	1.0	83.8
ycf2-trnI-CAU_p4	CCAGGATTCGAGTGGATGTC	GTTGGGCGCTTTAACCATTC	55.0	50.0	57.8	58.0	198 bp	1.0	83.7
ycf2-trnI-CAU_p5	TCCAGGATTCGAGTGGATGT	AGTTGGGCGCTTTAACCATT	50.0	45.0	58.1	58.4	200 bp	1.0	81.6