Hotspot

rpl32-trnL-UAG

SSC · 108723-108950 · 228 bp

Back to Symphytum

Pi 0.0136
MarkerSeek score 55.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
228
variable_sites
3
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGTTTTGTTGTGCGACAAACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 57.7 57.9 289-297 bp 1.0 71.7
rpl32-trnL-UAG_p2 AGTTTTGTTGTGCGACAAACA AGCAGCGTGTCTACCAATTT 38.1 45.0 57.7 57.8 282-290 bp 1.0 71.3
rpl32-trnL-UAG_p3 AGTTTTGTTGTGCGACAAACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 57.7 57.8 288-296 bp 1.0 71.1
rpl32-trnL-UAG_p4 AGTTTTGTTGTGCGACAAACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 57.7 57.8 287-295 bp 1.0 71.1
rpl32-trnL-UAG_p5 AGTTTTGTTGTGCGACAAACA GAGCAGCGTGTCTACCAATT 38.1 50.0 57.7 58.3 283-291 bp 1.0 70.6