Hotspot

rrn16-trnI-GAU

IRb · 97638-97926 · 289 bp

Back to Symphytum

Pi 0.0311

MarkerSeek score 70.40

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 289
variable_sites: 9
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	415-420 bp	1.0	78.0
rrn16-trnI-GAU_p2	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	383-388 bp	1.0	78.0
rrn16-trnI-GAU_p3	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	437-442 bp	1.0	77.9
rrn16-trnI-GAU_p4	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	405-410 bp	1.0	77.8
rrn16-trnI-GAU_p5	AAGTCGTAACAAGGTAGCCG	CTATCCATGTGGCTGAGAGC	50.0	55.0	57.6	57.8	424-429 bp	1.0	77.5