Hotspot

trnI-CAU-ycf2

IRb · 82388-82526 · 139 bp

Back to Symphytum

Pi 0.0458

MarkerSeek score 83.10

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 139
variable_sites: 6
pi: 0.0458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TCCAGGATTCGAGTGGATGT	50.0	50.0	58.0	58.1	199 bp	1.0	84.5
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	AGGATTCGAGTGGATGTCCT	50.0	50.0	58.0	57.8	196 bp	1.0	84.3
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	CAGGATTCGAGTGGATGTCC	50.0	55.0	58.0	57.8	197 bp	1.0	83.8
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	CCAGGATTCGAGTGGATGTC	50.0	55.0	58.0	57.8	198 bp	1.0	83.7
trnI-CAU-ycf2_p5	AGTTGGGCGCTTTAACCATT	TCCAGGATTCGAGTGGATGT	45.0	50.0	58.4	58.1	200 bp	1.0	81.6