Hotspot

ndhC-trnV-UAC

LSC · 49140-49912 · 773 bp

Back to Symphytum

Pi 0.0142

MarkerSeek score 63.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 11
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAACTCCTGAAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.8	58.1	908-909 bp	1.0	78.6
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	919-920 bp	1.0	77.4
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCTGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	910-911 bp	1.0	76.7
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCTG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.6	58.1	911-912 bp	1.0	76.7
ndhC-trnV-UAC_p5	CGGGGCTAAAACTCCTGAAA	AGTGCATGTTGGGTCTTTGA	50.0	45.0	57.8	57.6	972-973 bp	1.0	76.4