Hotspot

rpoC1-rpoB

LSC · 22974-23005 · 32 bp

Back to Symphytum

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 32
variable_sites: 1
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GGGTTTTGTCACCTCTCCAA	CGGAGGATGCTCCAGAATCT	50.0	55.0	57.6	59.0	271 bp	1.0	69.8
rpoC1-rpoB_p2	GCCCAAGCGCTTATTTGTTG	CGGAGGATGCTCCAGAATCT	50.0	55.0	58.7	59.0	222 bp	1.0	67.5
rpoC1-rpoB_p3	GGGTTTTGTCACCTCTCCAA	GGAGGATGCTCCAGAATCTT	50.0	50.0	57.6	56.7	270 bp	1.0	66.9
rpoC1-rpoB_p4	GGGTTTTGTCACCTCTCCAA	GGAGGATGCTCCAGAATCTTT	50.0	47.6	57.6	57.4	270 bp	1.0	64.8
rpoC1-rpoB_p5	CCCAAGCGCTTATTTGTTGA	CGGAGGATGCTCCAGAATCT	45.0	55.0	57.0	59.0	221 bp	1.0	64.7