Hotspot

atpI-rps2

LSC · 14723-14920 · 198 bp

Back to Symphytum

Pi 0.0253

MarkerSeek score 67.00

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 5
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GGAAACCCCCTATTTGCCAA	TTCCAGCAAATGATGACGCT	50.0	45.0	58.1	58.2	399 bp	1.0	79.2
atpI-rps2_p2	GTTGACCTACTTCCACACCG	TTCCAGCAAATGATGACGCT	55.0	45.0	58.2	58.2	372 bp	1.0	78.0
atpI-rps2_p3	AAGTACTTGACCGTGGACCT	TTCCAGCAAATGATGACGCT	50.0	45.0	58.3	58.2	419 bp	1.0	77.3
atpI-rps2_p4	GGAAACCCCCTATTTGCCAA	TGTGAAGGGCGTTCTAGCTA	50.0	50.0	58.1	58.4	334 bp	1.0	77.2
atpI-rps2_p5	GGAAACCCCCTATTTGCCAA	TTTGTGAAGGGCGTTCTAGC	50.0	50.0	58.1	58.5	336 bp	1.0	76.9