Hotspot

rpl23

LSC · 88596-88880 · 285 bp

Back to Symphoricarpos

Pi 0.014

MarkerSeek score 46.20

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: LSC
length_bp: 285
variable_sites: 4
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	ACCCGTCCTCTTCTCTTTGA	CCTACTGGATGCATGCCAAT	50.0	50.0	58.0	58.0	1138-1139 bp	1.0	81.0
rpl23_p2	ACCCGTCCTCTTCTCTTTGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	1187-1188 bp	1.0	81.0
rpl23_p3	CAATTCGCCTCTGACCATCA	CCTACTGGATGCATGCCAAT	50.0	50.0	58.0	58.0	1173-1174 bp	1.0	80.9
rpl23_p4	CGCCTCTGACCATCAAATGA	CCTACTGGATGCATGCCAAT	50.0	50.0	58.0	58.0	1168-1169 bp	1.0	80.9
rpl23_p5	TCGCCTCTGACCATCAAATG	CCTACTGGATGCATGCCAAT	50.0	50.0	58.0	58.0	1169-1170 bp	1.0	80.9