Hotspot

ycf1-trnN-GUU

IRa · 131380-132990 · 1611 bp

Back to Symphoricarpos

Pi 0

MarkerSeek score 62.30

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1611
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	TGAAATCGTAGGTGAAATGA	CCGAACGCTCTACCACTAAG	35.0	55.0	52.2	57.8	1573-1674 bp	1.0	44.0
ycf1-trnN-GUU_p2	AGTCATGAGATTATATTCCTCCCT	CCGAACGCTCTACCACTAAG	37.5	55.0	56.2	57.8	1627-1728 bp	1.0	44.0
ycf1-trnN-GUU_p3	TGAAATCGTAGGTGAAATGAA	CCGAACGCTCTACCACTAAG	33.3	55.0	53.2	57.8	1573-1674 bp	1.0	44.0
ycf1-trnN-GUU_p4	TTGAAATCGTAGGTGAAATGA	CCGAACGCTCTACCACTAAG	33.3	55.0	53.2	57.8	1574-1675 bp	1.0	44.0
ycf1-trnN-GUU_p5	TGAAATCGTAGGTGAAATGA	CCCCAAGTAGGATTCGAACC	35.0	55.0	52.2	57.7	1613-1714 bp	1.0	44.0