Hotspot

rpl2-rpl23

LSC · 88578-88595 · 18 bp

Back to Symphoricarpos

Pi 0.0556

MarkerSeek score 68.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TGCTCGGGGTAGAAGTTTTG	CACCTCTTAGAAAGAAAAGAACT	50.0	34.8	57.8	54.3	81 bp	1.0	41.0
rpl2-rpl23_p2	GCTCGGGGTAGAAGTTTTGT	CACCTCTTAGAAAGAAAAGAACT	50.0	34.8	57.8	54.3	80 bp	1.0	41.0
rpl2-rpl23_p3	GTGCTCGGGGTAGAAGTTTT	CACCTCTTAGAAAGAAAAGAACT	50.0	34.8	57.8	54.3	82 bp	1.0	41.0
rpl2-rpl23_p4	TGCTCGGGGTAGAAGTTTTG	CACCTCTTAGAAAGAAAAGAACTT	50.0	33.3	57.8	55.0	81 bp	1.0	41.0
rpl2-rpl23_p5	GCTCGGGGTAGAAGTTTTGT	CACCTCTTAGAAAGAAAAGAACTT	50.0	33.3	57.8	55.0	80 bp	1.0	41.0